exosome tem easy kit Search Results


total exosome rna isolation kit  (Thermo Fisher)
96
Thermo Fisher total exosome rna isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Total Exosome Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/Total+Exosome+Isolation+Kit/pmc07118280-133-19-24
Average 96 stars, based on 1 article reviews
total exosome rna isolation kit - by Bioz Stars, 2026-09
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protein isolation kit  (Thermo Fisher)
97
Thermo Fisher protein isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Protein Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/Total+Exosome+RNA+%26+Protein+Isolation+Kit/pmc06006291-288-16-19
Average 97 stars, based on 1 article reviews
protein isolation kit - by Bioz Stars, 2026-09
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total rna isolation kit  (Norgen Biotek)
96
Norgen Biotek total rna isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Total Rna Isolation Kit, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/Exosomal+RNA+Isolation+Kit/pmc05571886-34-17-23
Average 96 stars, based on 1 article reviews
total rna isolation kit - by Bioz Stars, 2026-09
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purification kit  (Norgen Biotek)
99
Norgen Biotek purification kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Purification Kit, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
purification kit - by Bioz Stars, 2026-09
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bca protein assay kit  (Thermo Fisher)
99
Thermo Fisher bca protein assay kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/BCA+Protein+Assay+Kit/pmc08645082-177-9-13
Average 99 stars, based on 1 article reviews
bca protein assay kit - by Bioz Stars, 2026-09
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exosome isolation kit  (EZBioscience)
90
EZBioscience exosome isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Exosome Isolation Kit, supplied by EZBioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/exosome+rna+purification+kit/ppr0548110-77-3-5
Average 90 stars, based on 1 article reviews
exosome isolation kit - by Bioz Stars, 2026-09
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cell culture media extracellular vesicle purification kits  (Norgen Biotek)
94
Norgen Biotek cell culture media extracellular vesicle purification kits
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Cell Culture Media Extracellular Vesicle Purification Kits, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/Cell+Culture+Media+Exosome+Purification+and+RNA+Isolation+Mini+Kit/pmc08999736-49-35-42
Average 94 stars, based on 1 article reviews
cell culture media extracellular vesicle purification kits - by Bioz Stars, 2026-09
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sortevtm ev-rna low volume enrichment kit  (Exosomics Siena)
90
Exosomics Siena sortevtm ev-rna low volume enrichment kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sortevtm Ev Rna Low Volume Enrichment Kit, supplied by Exosomics Siena, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sortevtm ev-rna low volume enrichment kit - by Bioz Stars, 2026-09
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micro bca protein assay kit  (Bio-Techne corporation)
91
Bio-Techne corporation micro bca protein assay kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Micro Bca Protein Assay Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/BCA+Protein+Assay+Kit/pmc09353410-204-28-25
Average 91 stars, based on 1 article reviews
micro bca protein assay kit - by Bioz Stars, 2026-09
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exosure exosome isolation kit  (Genecopoeia)
92
Genecopoeia exosure exosome isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Exosure Exosome Isolation Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosome+tem+easy+kit/ExoSure+Exosome+Isolation+Kit/custom%40ep002%4040767021
Average 92 stars, based on 1 article reviews
exosure exosome isolation kit - by Bioz Stars, 2026-09
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exosome isolation kit  (AMS Biotechnology)
98
AMS Biotechnology exosome isolation kit
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Exosome Isolation Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
exosome isolation kit - by Bioz Stars, 2026-09
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exosomes  (Beyotime)
99
Beyotime exosomes
Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide <t>RNA</t> (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations <t>in</t> <t>ΔPGRN-H69</t> analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Exosomes, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide RNA (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations in ΔPGRN-H69 analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Neoplasia (New York, N.Y.)

Article Title: Liver fluke granulin promotes extracellular vesicle-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma

doi: 10.1016/j.neo.2020.02.004

Figure Lengend Snippet: Programmed CRISPR/Cas9 mutation of the human progranulin gene. Panel A, linear map of a pre-designed lentiviral CRISPR/Cas9 vector containing fused codon-optimized puromycin resistance marker (dark gray bar)-Cas9 (yellow bar) and green fluorescent protein (green bar) driven by the mammalian elongation factor alpha-1 promoter (dark blue arrow); guide RNA (gRNA) targeting human granulin exon 2 (red bar) is expressed from a single vector. The human U6 promoter (blue arrow) drove the gRNA. The vector backbone includes the 5′- and 3′-long terminal repeats (LTR) of the HIV-1 provirus (light gray blocks). Panel B, schematic representation of the partial human granulin gene precursor, huPGRN on chromosome 17: NC_000017.11 regions 44,345,086–44,353,106 (8021 bp) and protein structure. Nucleotide sequence in exon 2 encodes the N-terminus and part of the granulin/epithelin module (GEM) of progranulin; indicating locations of gRNA (4417–4438 nt; red colored-letter) predicted double-stranded break (DSB) (red arrow). Panels C–E, on target INDEL mutations in ΔPGRN-H69 analysis by NGS libraries and CRISPResso bioinformatic platform. Frequency distribution of position-dependent insertions (red bars) (C) and deletion (magenta) (D) the major INDELs; 6 and 2 bp deletions at the programmed CRISPR/Cas9 cleavage site. Other minor mutations including 1 bp insertion (red square) or base substitution (bold) were observed further (>10 bp) predicted cleavage site (E). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Total RNA and evRNA either from H69 or ΔhuPGRN-H69 cells were isolated using RNAzol (Molecular Research Center, Inc.) or total exosome RNA isolation kit (ThermoFisher) following the manufacturer’s instructions.

Techniques: CRISPR, Mutagenesis, Plasmid Preparation, Marker, Sequencing